Synonym/Acronym:
Antinuclear antibodies (ANA), anti-DNA (anti-ds DNA), antiextractable nuclear antigens (anti-ENA, ribonucleoprotein [RNP], Smith [Sm], SS-A/Ro, SS-B/La), anti-Jo (antihistidyl transfer RNA [tRNA] synthase), and antiscleroderma (progressive systemic sclerosis [PSS] antibody, Scl-70 antibody, topoisomerase I antibody).

Common Use:
To diagnose multiple systemic autoimmune disorders; primarily used for diagnosing systemic lupus erythematosus (SLE).

Specimen:
Serum (3 mL) collected in a red-top tube.

Normal Findings:
(Method: Indirect fluorescent antibody for ANA and anticentromere; Immunoassay multiplex flow for anti-DNA, ENA, Scl-70, and Jo-1).

ANA and anticentromere: Titer of 1:40 or less. Anti-ENA, Jo-1, and anti-Scl-70: Negative. Reference ranges for anti-DNA, anti-ENA, anti-Scl-70, and anti-Jo-1 vary widely due to differences in methods and the testing laboratory should be consulted directly.