General

Synonym/Acronym:
antinuclear antibodies (ANA), anti-DNA (anti-ds DNA), antiextractable nuclear antigens (anti-ENA, ribonucleoprotein [RNP], Smith [Sm], SS-A/Ro, SS-B/La), anti-Jo (antihistidyl transfer RNA [tRNA] synthase), antiscleroderma (progressive systemic sclerosis [PSS] antibody, Scl-70 antibody, topoisomerase I antibody).

Rationale
To diagnose multiple systemic autoimmune disorders; primarily used for diagnosing systemic lupus erythematosus (SLE).

Patient Preparation
There are no food, fluid, activity, or medication restrictions unless by medical direction.

Normal Findings
Method: ELISA IgG for ANA or indirect fluorescent antibody (IFA) with human epithelial (HEp-2) substrate, IgG titer and pattern; Multiplex immunoassay for anti-DNA, anti-ENA, anti-Scl-70, and anti-Jo-1.

ANA: Titer of 1:80 or less. Anti-DNA, anti-ENA, anti-Jo-1, and anti-Scl-70: Negative. Reference ranges vary widely due to differences in methods, and the testing laboratory should be consulted directly.

Critical Findings and Potential Interventions
N/A