Testosterone, Total and Free

General

Synonym/Acronym:
N/A

Rationale
To evaluate testosterone to assist in identification of disorders related to early puberty, late puberty, and infertility while assessing gonadal and adrenal function.

Patient Preparation
There are no food, fluid, activity, or medication restrictions unless by medical direction.

Normal Findings
Method: HPLC/tandem MS for total testosterone and equilibrium dialysis for free testosterone.

Total Testosterone

Age	Conventional Units	SI Units (Conventional Units × 0.0347)
Newborn
Male	75–400 ng/dL	2.6–13.9 nmol/L
Female	16–64 ng/dL	0.56–2.22 nmol/L
1–5 mo
Male	Less than 360 ng/dL	Less than 12.49 nmol/L
Female	Less than 20 ng/dL	Less than 0.69 nmol/L
6–11 mo
Male	Less than 40 ng/dL	Less than 1.39 nmol/L
Female	Less than 9 ng/dL	Less than 0.31 nmol/L
1–5 yr
Male and female	Less than 20 ng/dL	Less than 0.69 nmol/L
6–7 yr
Male	Less than 20 ng/dL	Less than 0.69 nmol/L
Female	Less than 7 ng/dL	Less than 0.24 nmol/L
8–11 yr
Male	2–165 ng/dL	0.07–5.72 nmol/L
Female	1–30 ng/dL	0.04–1 nmol/L
12–13 yr
Male	Less than 600 ng/dL	Less than 20.8 nmol/L
Female	Less than 50 ng/dL	Less than 1.74 nmol/L
14–15 yr
Male	15–700 ng/dL	0.52–24.29 nmol/L
Female	Less than 60 ng/dL	Less than 2.08 nmol/L
Adult
Male	300–1080 ng/dL	10.41–37.48 nmol/L
Female	10–60 ng/dL	0.35–2.08 nmol/L
Older adult
Male	300–720 ng/dL	10.41–24.98
Female	5–32 ng/dL	0.17–1.11
Postmenopausal levels are about half the normal adult level for females; levels in women who are pregnant are three to four times the normal adult level for females who are not pregnant.

Free Testosterone

Age	Conventional Units	SI Units (Conventional Units × 3.47)
7 mo–9 yr
Male and female	Less than 2 pg/mL	Less than 7 pmol/L
10–11 yr
Male	Less than 7 pg/mL	Less than 25 pmol/L
Female	Less than 4 pg/mL	Less than 14 pmol/L
12–13 yr
Male	Less than 100 pg/mL	Less than 347 pmol/L
Female	Less than 7 pg/mL	Less than 25 pmol/L
14–15 yr
Male	4–140 pg/mL	13.88–486 pmol/L
Female	Less than 10 pg/mL	Less than 34.7 pmol/L
16–17 yr
Male	38–173 pg/mL	132–600 pmol/L
Female	Less than 10 pg/mL	Less than 34.7 pmol/L
Adult
Male	50–224 pg/mL	173.5–777.28 pmol/L
Female	1–9 pg/mL	3.47–31.23 pmol/L
Postmenopausal	1–6 pg/mL
Older adult
Male	5–75 pg/mL	17.35–260.25 pmol/L
Female	1–8.5 pg/mL	3.47–29.5 pmol/L

Critical Findings and Potential Interventions
N/A

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