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Red blood cell osmotic fragility, OF.
To assess the fragility of erythrocytes related to red blood cell lysis toward diagnosing diseases such as hemolytic anemia.
Whole blood collected in a green-top (heparin) tube and two peripheral blood smears.
(Method: Spectrophotometry) Hemolysis (unincubated) begins at 0.5% sodium chloride (NaCl) solution and is complete at 0.3% NaCl solution. Results are compared to a normal curve.