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Pleural Fluid Analysis

Pleural Fluid Analysis is a topic covered in the Davis's Lab & Diagnostic Tests.

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General

Synonym/Acronym:
Thoracentesis fluid analysis.

Common Use:
To assess and categorize fluid obtained from within the pleural space for infection, cancer, and blood as well as identify the cause of its accumulation.

Specimen:
Pleural fluid collected in a green-top (heparin) tube for amylase, cholesterol, glucose, lactate dehydrogenase (LDH), pH, protein, and triglycerides; lavender-top (EDTA) tube for cell count; sterile containers for microbiology specimens; of fluid in a clear container with anticoagulant for cytology. Ensure that there is an equal amount of fixative and fluid in the container for cytology.

Normal Findings:
(Method: Spectrophotometry for amylase, cholesterol, glucose, LDH, protein, and triglycerides; ion-selective electrode for pH; automated or manual cell count; macroscopic and microscopic examination of cultured microorganisms; microscopic examination of specimen for microbiology and cytology)

AppearanceClear
ColorPale yellow
AmylaseParallels serum values
CholesterolParallels serum values
CEAParallels serum values
GlucoseParallels serum values
LDHLess than 200 units/L
Fluid LDH-to-serum LDH ratio0.6 or less
Protein3 g/dL
Fluid protein-to-serum protein ratio0.5 or less
TriglyceridesParallel serum values
pH7.37–7.43
RBC countNone seen
WBC countLess than 1,000 cells/microL
CultureNo growth
Gram stainNo organisms seen
CytologyNo abnormal cells seen
CEA = carcinoembryonic antigen; LDH = lactate dehydrogenase; RBC = red blood cell; WBC = white blood cell.

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Citation

Bladh, Mickey Lynn., and Anne M. Van Leeuwen. "Pleural Fluid Analysis." Davis's Lab & Diagnostic Tests, 7th ed., F.A. Davis Company, 2017. Nursing Central, nursing.unboundmedicine.com/nursingcentral/view/Davis-Lab-and-Diagnostic-Tests/425300/all/Pleural_Fluid_Analysis.
Bladh ML, Van Leeuwen AM. Pleural Fluid Analysis. Davis's Lab & Diagnostic Tests. 7th ed. F.A. Davis Company; 2017. https://nursing.unboundmedicine.com/nursingcentral/view/Davis-Lab-and-Diagnostic-Tests/425300/all/Pleural_Fluid_Analysis. Accessed March 19, 2019.
Bladh, M. L., & Van Leeuwen, A. M. (2017). Pleural Fluid Analysis. In Davis's Lab & Diagnostic Tests. Available from https://nursing.unboundmedicine.com/nursingcentral/view/Davis-Lab-and-Diagnostic-Tests/425300/all/Pleural_Fluid_Analysis
Bladh ML, Van Leeuwen AM. Pleural Fluid Analysis [Internet]. In: Davis's Lab & Diagnostic Tests. F.A. Davis Company; 2017. [cited 2019 March 19]. Available from: https://nursing.unboundmedicine.com/nursingcentral/view/Davis-Lab-and-Diagnostic-Tests/425300/all/Pleural_Fluid_Analysis.
* Article titles in AMA citation format should be in sentence-case
TY - ELEC T1 - Pleural Fluid Analysis ID - 425300 A1 - Bladh,Mickey Lynn, AU - Van Leeuwen,Anne M, BT - Davis's Laboratory & Diagnostic Tests UR - https://nursing.unboundmedicine.com/nursingcentral/view/Davis-Lab-and-Diagnostic-Tests/425300/all/Pleural_Fluid_Analysis PB - F.A. Davis Company ET - 7 DB - Nursing Central DP - Unbound Medicine ER -